Protein ladder sequencing

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Protein ladder sequencing.

A new approach to protein sequencing is described. It consists of two steps: (i) ladder-generating chemistry, the controlled generation from a polypeptide chain by wet chemistry of a family of sequence-defining peptide fragments, each differing from the next by one amino acid; and (ii) data readout, a one-step readout of the resulting protein sequencing ladder by matrix-assisted laser-desorptio...

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ژورنال

عنوان ژورنال: Science

سال: 1993

ISSN: 0036-8075,1095-9203

DOI: 10.1126/science.8211132